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Image Search Results
Journal: Developmental cell
Article Title: Exo70 Generates Membrane Curvature for Morphogenesis and Cell Migration
doi: 10.1016/j.devcel.2013.07.007
Figure Lengend Snippet: (A) Molecular dynamics simulation of modeled Exo70 anti-parallel (left) and parallel (right) dimers on a DOPC/DOPS bilayer. Top and side views of the simulation snapshots show negative curvature formed in both directions. All snapshots were rendered with VMD (Humphrey et al., 1996). See also Supplemental Movie 2 for a more comprehensive view and Supplemental Movie 3 for analysis of negative curvature recorded in the simulations. (B) Comparison of the radius of curvature estimates (here R = 〈2Hmax〉−1) for different Exo70 variants on the membrane. Dashed line at 53.4 nm represents the threshold above which no inward membrane tubulation was detected in mesoscale simulations. (C) Snapshots from mesoscale simulations of inward growing tubules for model Exo70 domains at different surface concentration (from 20% to 100%); a0=14 nm, H0||=−1.0/a0 and εLL =1kBT. The extent of tubulation is Exo70 concentration-dependent. Exo70 proteins on the outer surface of the membrane are not shown in order to enhance the visibility of the tubules. See also Supplemental Figure 1.
Article Snippet:
Techniques: Comparison, Membrane, Concentration Assay